Application

Anti-GlucokinaseAntibody, clone 9G1.2 is an antibody against GCK for use in Western Blotting, Immunohistochemistry (Paraffin).

Research CategorySignaling

Research Sub CategoryGlucose/Glycogen Metabolism

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Glucokinase/GCK in human liver, colon, and tonsil tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glucokinase (EC 2.7.1.2; UniProt P35557; also known as GK, Hexokinase-4, Hexokinase-D, Hexokinase type IV, HK IV, HK4) is encoded by the GCK (also known as HHF3, MODY2) gene (Gene ID 2645) in human. Glucokinase/GK functions as the body′s primary glucose sensor by catalyzing the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, the rate-limiting step of glucose metabolism in human pancreas and liver. GK activity is regulated rapidly in response to the elevated blood glucose after feeding, whereby a change in GK conformation causes a dissociation of the nuclear GK-glucokinase regulatory protein (GKRP) complex, allowing GK translocation to the cytoplasm in an activated state. In mammals, there exist four types of hexokinases, HK1, HK2, and HK3 have high affinity for glucose, while GK (also known as HK4) has low affinity for glucose and its expression is restricted to the pancreas and liver. GCK gene mutations cause Maturity-onset diabetes of the young 2 (MODY2) and Familial hyperinsulinemic hypoglycemia 3 (HHF3).

Immunogen

GST-tagged recombinant internal fragment from the C-terminal half of human Glucokinase/GCK.

Epitope: Internal (C-terminal half).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glucokinase/GCK in 10 µg of HepG2 cell lysate.

Specificity

Clone 9G1.2 recognizes an epitope present in all three spliced isoforms of human Glucokinase/GCK reported by UniProt (P35557).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~52 kDa observed. ~52 kDa observed. 52.19 kDa (isoform 1), 52.13 kDa (isoform 2), and 52.04 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.